Gel filtration column for purification and desalting of proteins and nanoparticles larger than 25 kD. Processes sample volumes of 1.0 mL.
CentriPure PF10 Gel Filtration Columns are designed for rapid desalting and efficient removal of small molecules (dyes, salts, buffers, biotin, haptens, etc.) from proteins in a sample volume of 1.0 mL. The PF10 is also suitable for nanoparticle purification.
Purified antibodies, enzymes and other proteins larger than 25 kD are eluted using gravity into a buffer of choice. The removal efficiency of small molecules typically exceeds 99.99%, with a 1.5-fold dilution and a protein recovery generally greater than 85%. The sample is processed in under 15 minutes.
The gel matrix in CentriPure PF10 is Zetadex-50, a size-exclusion gel with an effective pore size of about 25 kD. Molecules larger than the pores are excluded from the gel, remain in the void volume, rapidly pass through the column, and are eluted free of small molecular weight contaminants.