CentriPure PF100 Columns
Gel filtration column for purification and desalting of proteins and nanoparticles larger than 25 kD. Processes sample volumes of 10 mL.
CentriPure PF100 Gel Filtration Columns are designed for rapid desalting and efficient removal of small molecules (dyes, salts, buffers, biotin, haptens, etc.) from proteins in a sample volume of 10.0 mL. The PF100 is also suitable for nanoparticle purification.
Purified antibodies, enzymes and other proteins larger than 25 kD are eluted using gravity into a buffer of choice. The removal efficiency of small molecules typically exceeds 99.99%, with a 1.5-fold dilution and a protein recovery generally greater than 85%. The sample is processed in under 15 minutes.
The gel matrix in CentriPure PF100 is Zetadex-50, a size-exclusion gel with an effective pore size of about 25 kD. Molecules larger than the pores are excluded from the gel, remain in the void volume, rapidly pass through the column, and are eluted free of small molecular weight contaminants.
Brand | |
---|---|
Manufacturer | |
Unit | |
Pack size |
You may also like…
-
CentriPure PF50 Columns
Gel filtration column for purification and desalting of proteins and nanoparticles larger than 25 kD. Processes sample volumes of 5 mL. CentriPure PF50 Gel Filtration Columns […]
-
CentriPure P100 Columns
Gel filtration column for purification and desalting of proteins larger than 5 kD. Processes sample volumes of 10 mL. CentriPure P100 Gel Filtration Columns are designed […]
-
CentriPure P500 Columns
Gel filtration column for purification and desalting of proteins larger than 5 kD. Processes sample volumes of 50 mL. CentriPure P500 Gel Filtration Columns are designed […]