Size Exclusion (SEC)

WorkBeads 40 SEC, WorkBeads 40/100 SEC and WorkBeads 40/10000 SEC

High performance size exclusion chromatography media for laboratory and large scale separation of proteins. Made from agarose, well established and well-known in the biotech industry.

  • Excellent Resolution
  • Robust Separation Results Can be Achieved Across a Wide Range of Proteins & Conditions
  • Chemically Stable for Cleaning in Place

Media Description

All WorkBeads™ 40 SEC media are produced from agarose using a cross-linking method that results in a highly porous and physically stable agarose matrix. Agarose based matrices have been successfully used over decades in biotechnology research and in the industrial purification of proteins. Agarose is proven to be excellently compatible with natural biomolecules like proteins, DNA carbohydrates etc. The material shows minimal non-specific interaction due to hydrophilic nature of agarose. Unlike matrices made from synthetic polymers, agarose does not have micro pores that can contribute to local pH variations in the microenvironment in the column and distorted separations.

WorkBeads 40 SEC for Size Exclusion Chromatography has a high selectivity which means the protein peaks are well separated with greater distance from each other than comparable products made from synthetic polymers. This means that the media has capacity to separate proteins well even when using high proteins loadings.

Resolution is the combined effect of selectivity (distance between peaks) and efficiency (peak width, depending on particle size). Separation media based on agarose are well known for excellent selectivity. The narrow particle size distribution around 45 micrometer in combination with very rigidity results in a media that is easy to pack in columns with very high efficiency and good flow characteristics.

WorkBeads 40 SEC is designed for high performance protein separations under a variety of conditions. The high resolution that can be obtained makes bead ideal for both preparative work and process scale separation of proteins.

Separation Ranges

Product Separation Range kDalton Exclusion Limit kDalton
WorkBeads 40/100 SEC 10 – 150 150
WorkBeads 40 SEC 50-1200 1200
WorkBeads 40/10000 SEC Very large molecules and particles > 10 000

Ordering Information

Article Number Product Name Column Size / Volume
40 300 001 WorkBeads 40 SEC Bulk Media – 25 ml
40 300 003 WorkBeads 40 SEC Bulk Media – 300 ml
40 300 010 WorkBeads 40 SEC Bulk Media – 1 L
40 300 050 WorkBeads 40 SEC Bulk Media – 5 L
40 340 001 WorkBeads 40/100 SEC Bulk Media – 25 ml
40 340 003 WorkBeads 40/100 SEC Bulk Media – 300 ml
40 340 010 WorkBeads 40/100 SEC Bulk Media – 1 L
40 340 050 WorkBeads 40/100 SEC Bulk Media – 5 L
40 350 003 WorkBeads 40/10000 SEC Bulk Media – 300 ml
40 350 010 WorkBeads 40/10000 SEC Bulk Media – 1 L
40 350 050 WorkBeads 40/10000 SEC Bulk Media – 5 L
40 350 001 WorkBeads 40/10000 Bulk Media – 25 ml