WorkBeads Protein A

WorkBeads™ Protein A is a new affinity resin designed for the purification of monoclonal and polyclonal antibodies.

• Higher dynamic binding capacity for monoclonal antibodies; excellent recovery & high purity
• Stronger coupling chemistry; high pH stability and low leakage
• Reliable, reproducible and efficient

Product description
WorkBeads Protein A affinity chromatography media is produced from agarose using a cross-linking method that results in a highly porous and physically stable agarose matrix. Agarose matrices have successfully been used for many years in biotechnology research and in the industrial purification of proteins.

The recombinant protein A attached to the medium is developed by Medicago and produced in E. coli under conditions free of components of animal origin. The protein A is engineered to facilitate an oriented coupling to the matrix which allows high binding capacities for target proteins. The specificity of the recombinant protein A for the Fc region of IgG provides excellent purification. The high capacity, chemical stability and low ligand leakage make WorkBeads Protein A ideal for purification of mono- and polyclonal antibodies.

WorkBeads™ Protein A is designed to allow oriented coupling to the bead via multipoint attachment. This allows high utilization of the immobilized protein A resulting in high IgG binding conditions.

Bead has a dynamic binding capacity of typically more than 40 mg IgG/ml media under standard binding conditions (PBS, pH 7.4 and 3 minutes residence time).

Dependency of the dynamic binding capacity on residence time. Frontal analysis using 1 mg/ml human normal serum IgG was performed in a 6.6 x 100 mm glass column (from Diba, Cambridge, UK) in the presence of PBS, pH 7.4.