WorkBeads 40 ACT and WorkBeads 40/10000 ACT

WorkBeads™ 40 ACT and WorkBeads 40/10 000 ACT pre-activated separation media are produced from agarose using a cross-linking method that result in a highly porous and physically stable agarose matrix.

Agarose based beads have been successfully used for decades in biotechnology research and in the industrial purification of proteins. Agarose is proven to be compatible with natural biomolecules such as proteins, DNA, carbohydrates etc. The material shows minimal non specific interaction due to the hydrophilic nature of agarose. Unlike matrices made from synthetic polymers, agarose does not have micro pores that can contribute to local pH variations in the microenvironment in the column which lead to distorted separations.

WorkBeads 40 ACT and WorkBeads 40/10 000 ACT are activated with Bromohydrin. This activation method is proprietary and based upon well known chemistry and allows you to perform the coupling chemistry in aqueous solutions.

Matrix-OCH2CH(OH)CH2Br + Nucleophil (i.e. -SH, -NH2 or -OH) –> Matrix-OCH2CH(OH)CH2-Nu

WorkBeads 40 ACT and WorkBeads 40/10 000 ACT are supplied as an aqueous suspension with 20% ethanol as preservative. After washing, the gel is immediately ready for use. As no toxic chemicals are involved and the WorkBeads 40 ACT products are stable at room temperature the coupling procedure can, as long as your application so allows, easily be performed on your bench and at room temperature.

WorkBeads 40 ACT and WorkBeads 40/10 000 ACT are ready for use. Proteins or other molecules with free amino and sulfhydryl l groups will bind easily to. Just add the ligand to the suspension, stir and incubate over night. WorkBeads 40/10 000 ACT is ideal for coupling large entities like immunglobulins. The exclusion limit is more than 10000 kD.